Rating
Title	Abnormal regulation of renal kallikrein in experimental diabetes: effects of insulin on prokallikrein synthesis and activitation
Alternative Title	Effects of diabetes and insulin on renal kallikrein
Author	Jaffa, Ayad A. Miller, Donald H. Bailey, Graham S. Chao, Julie Margolius, Harry S. Mayfield, Ronald K.
Contributor(s)	Journal of Clinical Investigation (Publisher)
Date	1987-12
Affiliation/College	College of Medicine College of Graduate Studies
Department	Medicine Pharmacology
Subject.MeSH	Kallikreins Diabetes Mellitus, Experimental Insulin Hyperinsulinism Kidney Insulin, Isophane Trypsin Inhibitors Soybean Proteins Enzyme Activation Enzyme Precursors Rats, Sprague-Dawley Models, Animal
Subject.LCSH	Kallikrein Diabetes Streptozotocin Trypsin inhibitors Insulin Insulin shock Kidneys Enzyme activation Sprague Dawley rats Rats as laboratory animals American Society for Clinical Investigation
Description	Article written by researchers from the Departments of Medicine and Pharmacology, Medical University of South Carolina and the Veterans Administration Medical Center, Charleston, South Carolina. Published in the Journal of Clinical Investigation, December 1987, volume 80, pages 1651-1659. Includes abstract, references, tables, and diagrams.
Collection	MUSC Faculty Journal Articles
Contributing Institution	Waring Historical Library (MUSC)
Rights	Copyright 1987, American Society for Clinical Investigation (ASCI).
Language	English
Type	Text
Format	application/pdf
Media Type	Periodicals
Resource Identifier	fja_0042_1987
Abstract	The effects of streptozotocin (STZ) diabetes and insulin on regulation of renal kallikrein were studied in the rat. 1 and 2 wk after STZ injection, diabetic rats had reduced renal levels and urinary excretion of active kallikrein. Tissue and urinary prokallikrein levels were unchanged, but the rate of renal prokallikrein synthesis relative to total protein synthesis was reduced 3045% in diabetic rats. Treatment of diabetic rats with insulin prevented or reversed the fall in tissue level and excretion rate of active kallikrein and normalized prokallikrein syn- thesis rate. To further examine insulin's effects, nondiabetic rats were treated with escalating insulin doses to produce hyperinsulinemia. In these rats, renal active kallikrein increased. Although renal prokallikrein was not increased significantly by hyperinsulinemia, its synthesis was increased. As this was accompanied by proportionally increased total protein synthesis, relative kallikrein synthesis rate was not changed. Excretion of active kallikrein was unchanged, but prokallikrein excretion was markedly reduced. Therefore, increased tissue active kallikrein seen with hyperinsulinemia can be explained not only by increased synthesis but also by retention and increased activation of renal prokallikrein. These studies show that STZ diabetes produces an impairment in renal kallikrein synthesis and suggest that this disease state also impairs renal prokallikrein activation. The findings also suggest that insulin modulates renal kallikrein production, activation, and excretion.

Abnormal regulation of renal kallikrein in experimental diabetes: effects of insulin on prokallikrein synthesis and activitation

Description

Tags

Comments