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Title	Declining sensitivity to interleukin 3 of murine multipotential hemopoietic progenitors during their development: application to a culture system that favors blast cell colony formation
Alternative Title	Murine blast cell colonies
Author	Koike, Kenichi Ihle, James N. Ogawa, Makio
Contributor(s)	Journal of Clinical Investigation (Publisher)
Date	1986-03
Affiliation/College	College of Medicine
Department	Medicine
Subject.MeSH	Cell Cycle--drug effects Cells, Cultured Colony-Forming Units Assay Hematopoietic Stem Cells--drug effects Interleukin-3 Fluorouracil Lymphokines--pharmacology Mice Spleen--cytology
Subject.LCSH	Cells Cytology Cell culture Hematopoietic stem cells Interleukin-3 Fluorouracil Lymphokines Spleen Mice as laboratory animals American Society for Clinical Investigation
Description	Article written by researchers from the Department of Medicine, Medical University of South Carolina;Veterans Administration Medical Center, Charleston, South Carolina;and Biological Carcinogenesis Program, National Cancer Institute-Frederick Cancer Research Facility. Published in the Journal of Clinical Investigation, March 1986, volume 77, pages 894-899. Includes abstract, references, tables, diagrams, and black-and-white photographic illustrations.
Collection	MUSC Faculty Journal Articles
Contributing Institution	Waring Historical Library (MUSC)
Rights	Copyright 1986, American Society for Clinical Investigation (ASCI).
Language	English
Type	Text
Format	application/pdf
Media Type	Periodicals
Resource Identifier	fja_0047_1986
Abstract	When spleen cells of 5-fluorouracil (5-FU)-treated mice were cultured in the presence of interleukin 3 (IL-3), most colonies revealed IL-3 concentration-dependent colony formation except for mast cell colonies and blast cell colonies. While most colonies were smaller in lower concentrations of IL-3, the size of the blast cell colonies were similar between high and low IL-3 groups. These data suggested that blast cell colony development requires less IL-3 than the development of multilineage colonies from blast cell colonies. This notion was supported by experiments in which IL-3 was added twice to cultures of spleen cells of 5-FUtreated mice. When low concentrations of IL-3 were added on day 7, there was a reduction in the number of multilineage colonies formed without an effect on the number of blast cell colonies. Using this information, we developed a culture system that favors blast cell colony formation by cells of normal mice. When low (20 U/ml) concentrations of IL-3 were added to cultures of spleen cells of normal mice on day 7 of incubation in media containing 2-5% fetal calf serum, blast cell colonies were the predominant colony type. The blast cell colonies revealed high but variable secondary replating efficiencies. These data suggest that multipotential progenitors may become less sensitive to IL-3 as they differentiate in culture.

Declining sensitivity to interleukin 3 of murine multipotential hemopoietic progenitors during their development: application to a culture system that favors blast cell colony formation

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