Rating
Title	Low density lipoprotein metabolism by human macrophages activated with low density lipoprotein immune complexes : a possible mechanism of foam cell formation
Author	Griffith, R. L. Virella, G. T. Stevenson, H. C. Lopes-Virella, M. F.
Contributor(s)	Journal of Experimental Medicine (Publisher)
Date	1988-09
Affiliation/College	College of Graduate Studies College of Medicine
Department	Basic and Clinical Immunology and Microbiology Medicine
Subject.MeSH	Foam Cells Cholesterol Esters Macrophage Activation Receptors, LDL Antigen-Antibody Complex Macrophages Atherosclerosis Lipoproteins Coronary Disease
Collection	MUSC Faculty Journal Articles
Contributing Institution	Waring Historical Library (MUSC)
Rights	Copyright © 1988 The Rockefeller University Press. For more information contact the Waring Historical Library, Charleston, SC 29425.
Language	English
Type	Text
Format	application/pdf
Media Type	Articles
Resource Identifier	fja_1988_0003
Abstract	Human macrophages play a key role in atherogenesis and are believed to be the progenitors of the cholesteryl ester (CE)-laden foam cells present in early atherosclerotic lesions. Several mechanisms by which macrophages accumulate CE have been recently described. One involves a perturbation in LDL metabolism subsequent to macrophage activation. Thus, we decided to study the effect of macrophage activation by immune complexes on N-LDL metabolism. Initially, LDL-containing immune complexes (LDL-IC) were chosen, since increased plasma levels of these IC have been reported in patients with coronary heart disease. Human macrophages stimulated for 22 h with LDL-IC (250 micrograms/ml) and incubated afterwards for 20 h with 10 micrograms/ml 125I-N-LDL showed a six- and fourfold increase in the accumulation and degradation, respectively, of 125I-N-LDL over the values observed in nonstimulated cells. Scatchard analysis of 125I-N-LDL-specific binding suggests an increase (20-fold) in the number of LDL receptors in macrophages stimulated with LDL-IC. We studied other immune complexes varying in size and antigen composition. Some of the IC were able to stimulate, although to a lesser degree, the uptake of N-LDL by macrophages. Lipoprotein IC are more efficient and have the greatest capacity to increase N-LDL uptake and CE accumulation. We conclude that human macrophage activation by LDL-IC leads to an increase in LDL receptor activity and promotes in vitro foam cell formation.
Bibliographic Citation	Griffith, R. L., Virella, G. T., Stevenson, H. C., & Lopes-Virella, M. F. (1988). Low density lipoprotein metabolism by human macrophages activated with low density lipoprotein immune complexes: a possible mechanism of foam cell formation. The Journal of Experimental Medicine, 168(), 1041-1059: https://doi.org/10.1084/jem.168.3.1041

Low density lipoprotein metabolism by human macrophages activated with low density lipoprotein immune complexes : a possible mechanism of foam cell formation

Description

Tags

Comments