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Title	Biochemical characterization of a filtered synaptoneurosome preparation from guinea pig cerebral cortex : cyclic adenosine 3':5'-monophosphate- generating systems, receptors, and enzymes
Author	Hollingsworth, E. B. McNeal, E. T. Burton, J. L. Williams, R. J. Daly, J. W. Creveling, C. R.
Contributor(s)	Journal of Neuroscience (Publisher)
Date	1985-08
Affiliation/College	College of Graduate Studies
Department	Pathology and Laboratory Medicine
Subject.MeSH	Adenosine Guinea Pigs Adenylyl Cyclases Colforsin Histamine Cyclic AMP 2-Chloroadenosine Norepinephrine Synaptosomes Receptors, Histamine H1 Microscopy, Electron Cerebral Cortex Phosphoric Diester Hydrolases Neuroglia Centrifugation S100 Proteins
Collection	MUSC Faculty Journal Articles
Contributing Institution	Waring Historical Library (MUSC)
Rights	Copyright © 1985 The Society for Neuroscience. For more information contact the Waring Historical Library, Charleston, SC 29425.
Language	English
Type	Text
Format	application/pdf
Media Type	Articles
Resource Identifier	fja_1985_0004
Note	Article source publication: Hollingsworth, E. B., McNeal, E. T., Burton, J. L., Williams, R. J., Daly, J. W., & Creveling, C. R. (1985). Biochemical characterization of a filtered synaptoneurosome preparation from guinea pig cerebral cortex: cyclic adenosine 3':5'-monophosphate- generating systems, receptors, and enzymes. The Journal of Neuroscience, 5(8), 2240-2253: https://doi.org/10.1523/JNEUROSCI.05-08-02240.1985
Abstract	A particulate preparation was obtained by low speed centrifugation of guinea pig cerebral cortical homogenates prepared with a Krebs-Henseleit buffer. Light microscopic examination, using a reflected light differential interference contrast system, reveals the presence of intact neurons, axonal fragments, glial cells, and erythrocytes along with an abundance of small spherical entities (diameter about 1.1 micron) and snowman-shaped entities (diameter of larger sphere about 1.1 micron, diameter of attached smaller sphere about 0.6 micron). Many unattached smaller spherical entities are also present (diameter about 0.6 micron). Pressure filtration through 5- or 10-micron Millipore filters, followed by low speed centrifugation and resuspension, removes most of the larger entities to afford a suspension composed mainly of the small spherical and snowman-shaped entities. Electron microscopic examination reveals the presence of many synaptosomes with attached resealed postsynaptic entities. It is proposed that these correspond to the snowman-shaped entities to be termed synaptoneurosomes. Accumulations of cyclic AMP elicited by 2-chloroadenosine and histamine, and by combinations of 2-chloroadenosine, histamine, norepinephrine, and forskolin, are lower in filtered than in unfiltered preparations, whereas accumulations elicited by forskolin are unchanged. Levels of adenylate cyclase are reduced by filtration, whereas levels of phosphodiesterase are unchanged. Filtration reduces levels of markers for whole cells and endothelial cells, whereas neuronal markers such as acetylcholinesterase activity and norepinephrine uptake are increased. Levels of S-100 protein, a marker for glial cells, are not significantly decreased. There is no apparent change in the density of many receptors or ion channels. Levels of A1- adenosine and H1-histamine receptors are increased, whereas levels of so-called peripheral benzodiazepine-binding sites are decreased.

Biochemical characterization of a filtered synaptoneurosome preparation from guinea pig cerebral cortex : cyclic adenosine 3':5'-monophosphate- generating systems, receptors, and enzymes

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