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Title	Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages
Alternative Title	c-sis gene expression by human alveolar macrophages
Author	Mornex, Jean-Francois;Martinet, Yves;Yamauchi, Kohei;Bitterman, Peter B.;Grotendorst, Gary R.;Chytil-Weir, Anna;Martin, George R.;Crystal, Ronald G.
Contributor(s)	Journal of Clinical Investigation (Publisher)
Date	1986-07
Subject.MeSH	Chemotaxis Cycloheximide Gene Expression Regulation Hydrogen-Ion Concentration Lung Diseases Platelet-Derived Growth Factor Proto-Oncogenes Pulmonary Alveoli RNA, Messenger Radioimmunoassay Transcription, Genetic Trypsin Genetics Metabolism Inflammation
Subject.LCSH	Chemotaxis;Genetic regulation;Hydrogen-ion concentration;Lungs--Diseases;Platelet-derived growth factor;Proto-oncogenes;Pulmonary alveoli;Messenger RNA;Radioimmunoassay;Genetic transcription;Trypsin;Genetics;Metabolism;Inflammation;American Society for Clinical Investigation
Description	Article written by researchers from the Pulmonary Branch, National Heart, Lung, and Blood Institute, and Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health;and Division of Rheumatology and Immunology, Department of Medicine, Medical University of South Carolina. Published in the Journal of Clinical Investigation, July 1986, volume 78, pages 61-66. Includes abstract, references, and diagrams.
Collection	MUSC Faculty Journal Articles
Contributing Institution	Waring Historical Library (MUSC)
Date Digital	2015-09
Rights	Copyright 1986, American Society for Clinical Investigation (ASCI).
Language	English
Type	Text
Format	application/pdf
Media Type	Periodicals
Resource Identifier	fja_0054_1986
Abstract	Alveolar macrophages from normal individuals and patients with interstitial lung diseases spontaneously expressed a 4.2-kilobase mRNA complementary to the c-sis gene, a proto-oncogene coding for one of the chains of platelet-derived growth factor (PDGF). Concomitantly, these cells released a mediator with the properties of PDGF, including: (a) chemotactic factor for smooth muscle cells whose activity was resistant to heat and acid, but sensitive to reduction;(b) mitogenic (competence) activity for fibroblasts;(c) ability to compete with PDGF for its receptor, and (d) precipitated by an anti-PDGF antibody. While blood monocytes did not contain c-sis mRNA transcripts, monocytes matured in vitro expressed c-sis, consistent with the concept that expression of c-sis occurs during the differentiation of monocytes into alveolar macrophages. Together with the known actions of PDGF, these observations suggest that the c-sis proto-oncogene and its PDGF product are part of the armamentarium available to the alveolar macrophages for normal lung defense and participation in lung inflammation.

Spontaneous expression of the c-sis gene and release of a platelet-derived growth factorlike molecule by human alveolar macrophages

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